Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 117550-36-4 | Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | MFCD14680132 | 250mg
AstaTech | TERT-BUTYL 4-(CYANOMETHYL)PHENYLCARBAMATE | 1g | 449717503 | 21119 | 95.000 | 1233249-35-8 | MFCD17465121 | 232.283 | C13H16N2O2
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Sigma Aldrich Fine Chemicals Biosciences TRIS 3 HYDROXYPROPYLTRIAZOLY1G
Tris(3-hydroxypropyltriazolylmethyl)amine, 1g
S2 Media 1L 10x TRIS Buffered Saline (TBS, 10x), pH 7.4 in 1L Media Bottle, 1/each
S2Media™ 10x TRIS Buffered Saline (TBS, 10x), pH 7.4 is a ready to use stock buffer often used for immunodetection techniques, including Western blot protocols. To prepare a 1x solution, mix one volume of 10x solution with 9 volumes of 10x buffer solution.• Product meets reference methods and performance criteria.• TBS,10x should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions. • Product is 0.1µm filtered. Bottles are RNase, DNase, and protease free.
MOLTOX Molecular Toxicology Inc 20 mM Tris-HCL, pH 8.6, 1000 mL/bottle, Store at Room Temperature
MOLTOX manufactures a wide variety of buffers for your laboratory needs. All MOLTOX reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.0) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 8 0) - 2x1L (For Research Use and Further Manufacturing Only)
Biotang Inc Laemmli Buffer with PYRONINY, 4X Reducing, 25 ml
Laemmli Buffer with PYRONINY, 4X Reducing, 25 ml.
Alkali Scientific CellPro™ Tris Buffered Saline 20X, pH 7.4, 20L (modified)
Alkali Scientific™ CellPro™ TBS, 20X, pH 7.4, offers a sterile and concentrated Tris-buffered saline (TBS) solution, meticulously formulated to meet the demands of various molecular biology applications. With a pH of 7.4, this buffer provides optimal conditions for a wide range of biochemical and immunological assays, ensuring reliable and reproducible results. The 20X concentration allows for convenient dilution to the desired working strength, providing flexibility and efficiency in the laboratory. Sterilized to maintain purity and eliminate the risk of contamination, CellPro™ TBS is ready for immediate use in your research experiments. Trust in the quality and consistency of CellPro™ buffers to support your molecular biology endeavors, delivering accurate and precise results every time.
Bioworld Tricine-SDS Sample Buffer, Reducing (2X), 25 mL
Product DescriptionTricine-SDS is a sample buffer under reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, 0.01% phenol red, and 4% β-mercaptoethanol.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.